Flavone Glycoside Antagonizes Psilocybin-Induced Toxicity by Reducing Oxidative Stress in Rats Model

Objective To explore the effects of flavone glycoside on psilocybin-induced oxidative damage in rats.Methods Five group Sprague-Dawley rats(10 for each group) were received vehicle(Con),low dose of psilocybin(i.p.,0.5 μg/kg body weight)(LD),high dose of psilocybin(i.p.,1.5 μg/kg body weight)(HD),low dose of psilocybin(i.p.) plus flavone glycoside(i.p.100 mg/kg body weight)(LDR),and high dose of psilocybin(i.p.) plus flavone glycoside(i.p.)(HDR),respectively.Ratio of organ weight to body weight of each group was measured.Aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in serum of each group was evaluated with automated biochemistry analyzer.Oxidative stress-associated biochemical profile such as superoxide dismutase(SOD) and malondialdehyde(MDA) was assessed using kits.Liver histology of each group was observed using immunohistochemistry method.Results Compared with control group,AST and ALT in HD group were significantly increased,suggested that high dose of psilocybin induced toxic damage.After high dose treatment with psilocybins,MDA and SOD of liver homogenesis were elevated but this enhancement was reduced in HDR group,indicated that flavone glycoside alleviated psilocybin-induced oxidative stress.Interestingly,this significant increase of SOD was not observed in the kidney and heart lysates,implying that injured sites may be organ-dependent.Furthermore,histological analysis showed that high dose psilocybin induced liver damage which was reversed by flavone glycoside.Conclusion Oxidative damage induced by psilocybin could be attenuated by flavone glycoside.Our present study provided a novel mechanism of psilocybin-induced toxicity evidence and its antidote strategy.